Coding
TMM mk 2

Part:BBa_K4680001:Design

Designed by: Chase Tarrillo   Group: iGEM23_FSU   (2023-06-06)


Roseovarius sp. 217 TMM


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 736
    Illegal PstI site found at 1327
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 736
    Illegal PstI site found at 1327
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 736
    Illegal PstI site found at 1327
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 736
    Illegal PstI site found at 1327
    Illegal AgeI site found at 183
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Trimethylamine monooxygenase (TMM) is an enzyme found in marine bacteria. This specific strain comes from the Roseovarius sp 217 bacteria. These marine bacteria convert TMA into TMAO and then further convert to formaldehyde and ammonia. This TMM sequence was original taken from a previous AUC Turkey iGEM team but the FSU iGEM team revmoved the prefix and suffx from the sequence. Removing them would only leave us with the TMM coding sequence and was better for us to construct a biological device specific to our project.

Source

This entry is incomplete at this time.

References